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VDAC2和醛甲确定膜蛋白的K562 细胞表达增加下铁剥夺 导言 作为一项过渡金属，铁使电子传递和 氧化还原反应。由于这个能力，铁是必要的 所有活的生物体。铁是关键组成部分，许多 细胞蛋白，包括酶和调控蛋白 它所需的细胞过程如DNA 三磷酸腺苷的合成和生产[ 1 ， 2 ] 。 更高层次的蜂窝铁可导致细胞氧化 损伤和死亡。另一方面，缺铁 类似的情况使人缺氧，可诱导细胞凋亡 [ 3 ， 4 ] 。因此，处理铁内的细胞是 严格控制之下。蜂窝铁水平是通过控制 后转录调控的铁运输，储存， 和利用以及描述墨西哥革命制度党（铁规章 蛋白） /爱尔兰（铁反应元件）系统[ 5 ， 6 ] 。安大略 另一方面，铁，本身，似乎是参与转录 调控的几个基因[ 7-10 ] 。此外， 铁可以调节基因表达细胞通过氧化还原状况。 铁的影响转录通过铁产生 活性氧（ ROS ）和铁损耗模拟 缺氧[ 11-15 ] 。结果表明，铁剥夺 可能会导致缺氧诱导因子- 1α激活[ 4 ] 。 铁的能力产生活性氧可能代表 一个重要机制，它可以调节许多细胞 功能，包括铁矿石运输。就是铁铁减少 反应与超氧阴离子自由基，随后有色金属
Abstract Objective : Workers peripheral blood lymphocytes of p53 and DNA damage and repair gene mRNA expression levels of benzene exposure and the environment, explore specificity, sensitivity, stability, easy to collect specimens of the advantages of detecting indicators used benzene health care professional Methods : SYBR GreenI chimeric fluorescence quantitative real-time fluorescence RT-PCR analysis, testing workers exposed to 72 (under different posts divided into Group A, Group B) and 29 staff were peripheral blood lymphocytes and related gene p53 mRNA expression Application of differential expression analysis software REST 2005, compared with the control group operating multiple groups to express differences; While peripheral blood leukocytes, hemoglobin and platelet count; Sampling of raw materials toluene, benzene spraying agent content; Workplace air toluene concentration Results : Group A and Group B p53, p21, wild, Ape1 and Mdm2 expression levels compared with the control group was no significant difference, in Group A sword, Bcl-2, Bax, and Xpc Xpa expression level downward, Group B expression edged down compared with the control group was statistically significant A group of white blood cell, hemoglobin and platelet lower than the control group, Group B platelet lower than the control group differences are Conclusion : benzene-exposed workers part of the blood cells with DNA damage and repair gene mRNA expression levels of benzene and non-exposed control group SYBR GreenI chimeric fluorescent real-time RT-PCR and REST 2005 statistical software for detection and analysis of differentially expressed mRNA level, applies to occupational population of molecular epidemiological studies and health
这估计只能你不太懂的地方告诉告诉你 全文翻译的话该收费了都 或者看哪个比较清闲的人愿意吧 呵呵
铁铁在与过氧化物阴离子和后来亚铁的反应减少iron被氧化和因而电烙可能引起高度易反应 在Fenton反应的hydroxyl基础。 概略反应， 指Haber-Weiss反应，发生 在过氧化物的hydroxyl根本生产和减退 concentration [15]。 低分子大量铁复合体 能也的are导致ROS [16， 17]。 We以前展示了那铁剥夺 stimulates从铁枸橼酸盐的铁举起由人的erythroleukemia K562细胞和那这刺激依靠 protein综合。 然而，没有的介入任何知道 与一个建议的角色的proteins在非铁传递酶铁运输across查出了质膜[18]。 存在 铁举起的transport系统从低分子大量 complexes包括铁枸橼酸盐是有大量文件证明的[19– 23]。 然而，在运输介入的具体分子 system保持阴暗，即使证明of这些分子高度富挑战性主要归结于 在一些铁混乱的their潜在的介入。 为了辨认候选人膜分子 the运输系统，我们使用了两相分成 允许细胞膜分数的隔离的system by具体方式。 随后，我们使用了 high-resolution第2电泳法和计算机分析 蛋白质的for侦查与增加的水平的在膜 K562在铁剥夺之下的细胞的fraction。 蛋白质 增加的with平实被辨认了使用液体 chromatography与纵排质谱分析结合了。 We辨认了二这样蛋白质，即，二磷酸果糖酶A (ALDA)和电压依赖阴离子渠道2 (VDAC2)。 The二磷酸果糖酶A和VDAC2增加的表示 K562在铁剥夺之下的细胞被证实了 western污点分析。
分太少，工作量太大！
太佩服了。10分翻译这么多。楼主。多给人几分吧。
VDAC2和醛甲确定膜蛋白的K562 细胞表达增加下铁剥夺 导言 作为一项过渡金属，铁使电子传递和 氧化还原反应。由于这个能力，铁是必要的 所有活的生物体。铁是关键组成部分，许多 细胞蛋白，包括酶和调控蛋白 它所需的细胞过程如DNA 三磷酸腺苷的合成和生产[ 1 ， 2 ] 。 更高层次的蜂窝铁可导致细胞氧化 损伤和死亡。另一方面，缺铁 类似的情况使人缺氧，可诱导细胞凋亡 [ 3 ， 4 ] 。因此，处理铁内的细胞是 严格控制之下。蜂窝铁水平是通过控制 后转录调控的铁运输，储存， 和利用以及描述墨西哥革命制度党（铁规章 蛋白） /爱尔兰（铁反应元件）系统[ 5 ， 6 ] 。安大略 另一方面，铁，本身，似乎是参与转录 调控的几个基因[ 7-10 ] 。此外， 铁可以调节基因表达细胞通过氧化还原状况。 铁的影响转录通过铁产生 活性氧（ ROS ）和铁损耗模拟 缺氧[ 11-15 ] 。结果表明，铁剥夺 可能会导致缺氧诱导因子- 1α激活[ 4 ] 。 铁的能力产生活性氧可能代表 一个重要机制，它可以调节许多细胞 功能，包括铁矿石运输。就是铁铁减少 反应与超氧阴离子自由基，随后有色金属
Abstract Objective : Workers peripheral blood lymphocytes of p53 and DNA damage and repair gene mRNA expression levels of benzene exposure and the environment, explore specificity, sensitivity, stability, easy to collect specimens of the advantages of detecting indicators used benzene health care professional Methods : SYBR GreenI chimeric fluorescence quantitative real-time fluorescence RT-PCR analysis, testing workers exposed to 72 (under different posts divided into Group A, Group B) and 29 staff were peripheral blood lymphocytes and related gene p53 mRNA expression Application of differential expression analysis software REST 2005, compared with the control group operating multiple groups to express differences; While peripheral blood leukocytes, hemoglobin and platelet count; Sampling of raw materials toluene, benzene spraying agent content; Workplace air toluene concentration Results : Group A and Group B p53, p21, wild, Ape1 and Mdm2 expression levels compared with the control group was no significant difference, in Group A sword, Bcl-2, Bax, and Xpc Xpa expression level downward, Group B expression edged down compared with the control group was statistically significant A group of white blood cell, hemoglobin and platelet lower than the control group, Group B platelet lower than the control group differences are Conclusion : benzene-exposed workers part of the blood cells with DNA damage and repair gene mRNA expression levels of benzene and non-exposed control group SYBR GreenI chimeric fluorescent real-time RT-PCR and REST 2005 statistical software for detection and analysis of differentially expressed mRNA level, applies to occupational population of molecular epidemiological studies and health
分太少，工作量太大！
真核细胞的细胞核是最大的细胞器，细胞核对染色体组有保护作用（原核细胞的遗传物质存在于拟核中）。细胞核含有一或二个核仁，核仁促进细胞分裂。核膜贯穿许多小孔，小分子可以自由通过核膜，而象mRNA和核糖体等大分子必须通过核孔运输。Alleukaryotic cells contain most of the various kinds of organelles, and eachorganelle performs a specialized function in the Organelles described in this section includeribosomes, the endoplasmic reticulum, the Golgi complex, vacuoles, lysosomes,mitochondria, and the plastids of plant
Graduate as a Master of biochemicals and molecular biologyUndergraduate degree of biotechnology
充分理解这些聚合物获得性能的机制，需要在这个机制作用过程中的各个阶段捕捉聚合物的原子结构。这样应该很好懂了
VDAC2和醛甲确定膜蛋白的K562 细胞表达增加下铁剥夺摘要：我们已经表明以前铁剥夺大大刺激了摄取的非转铁铁铁柠檬酸的白血病K562细胞细胞和刺激，这取决于蛋白质的合成。然而，我们没有发现增加任何的表达众所周知铁转运蛋白（可伐学者等。 （ 2006 ）血细胞与分子杂志37:95-99 ） 。因此，为了确定膜蛋白的K562细胞表达增加铁剥夺下，我们采用了分离膜蛋白的两相分配制度， 蛋白质分离的高分辨率二维电泳， 计算机鉴别分析，并串联质谱。采用这些技术，我们确定了两个蛋白质与统计学上调，即： 醛甲（阿尔达）和电压依赖阴离子通道2 （ VDAC2 ） 。该上调醛缩酶A和VDAC2在K562细胞剥夺下铁也证实了免疫印迹分析。这是第一次当控制的醛缩酶A和VDAC2水平的铁营养状况的细胞是证明。
II类释放因子，与GDP结合时，还能约束大亚基。在原核生物中，RF3是II类释放因子。在真核生物中，eRF3是II类释放因子。在I类和II类释放因子结合后，I类释放因子的基序---甘氨酸谷氨酰胺（或称GGQ）从P位的tRNA触发蛋白水解从核糖体释放蛋白触发释放因子的回收。首先，GTP在II类释放因子上交换成GDP。这促使II类释放因子将I类释放因子从核糖体推开。
Abstract Objective : Workers peripheral blood lymphocytes of p53 and DNA damage and repair gene mRNA expression levels of benzene exposure and the environment, explore specificity, sensitivity, stability, easy to collect specimens of the advantages of detecting indicators used benzene health care professional Methods : SYBR GreenI chimeric fluorescence quantitative real-time fluorescence RT-PCR analysis, testing workers exposed to 72 (under different posts divided into Group A, Group B) and 29 staff were peripheral blood lymphocytes and related gene p53 mRNA expression Application of differential expression analysis software REST 2005, compared with the control group operating multiple groups to express differences; While peripheral blood leukocytes, hemoglobin and platelet count; Sampling of raw materials toluene, benzene spraying agent content; Workplace air toluene concentration Results : Group A and Group B p53, p21, wild, Ape1 and Mdm2 expression levels compared with the control group was no significant difference, in Group A sword, Bcl-2, Bax, and Xpc Xpa expression level downward, Group B expression edged down compared with the control group was statistically significant A group of white blood cell, hemoglobin and platelet lower than the control group, Group B platelet lower than the control group differences are Conclusion : benzene-exposed workers part of the blood cells with DNA damage and repair gene mRNA expression levels of benzene and non-exposed control group SYBR GreenI chimeric fluorescent real-time RT-PCR and REST 2005 statistical software for detection and analysis of differentially expressed mRNA level, applies to occupational population of molecular epidemiological studies and health