只有摘要，没有全文。 Formation of diacetyl by cell-free extracts of Leuconostoc lactis This article is not included in your organization's However, you may be able to access this article under your organization's agreement with EKieran N Jordana, , , Margie O'donoghueb, Seamus Condonb and timothy M Coganaa Teagasc, National Dairy Products Research Centre, Moorepark, Fermoy, C Cork, Irelandb Department of Microbiology, University College Cork, Cork, IrelandReceived 28 May 1996; revised 19 July 1996; accepted 14 August ; Available online 22 March AbstractDiacetyl formation was linear with time and with protein concentration when a cell-free extract of Leuconostoc lactis NCW1 was added to a buffer system containing pyruvate, thiamine pyrophosphate and MgS4 (final concentrations 60 mM, 11 mM and 22 mM, respectively) No diacetyl was detected in the absence of pyruvate or cell-free extract and no increase in diacetyl formation was detected on the addition of acetyl-CoA When 2-acetolactate (6 mM) was the substrate, autodecarboxylation to diacetyl and acetoin occurred under aerobic and anaerobic When cell-free extract was added, decarboxylation of 2-acetolactate to acetoin and diacetyl increased 4–6-fold, under aerobic and anaerobic When the cell-free extract was boiled, diacetyl formation from 2-acetolactate was reduced to the level of The results suggest that diacetyl is formed enzymatically in the presence and absence of oxygen, as well as spontaneously, from 2-
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