Column chromatography purification of the pigment in WolfberryAbstract: Chinese wolfberry has a "arrests restless Masushi, up five workers and injured seven, Zhuang Xin Qi, in addition to toxic heat, scattered sores, in addition to the wind eyesight," the Modern clinical studies show that wolfberry possess immunomodulatory, antitumor, anti-aging, anti-fatty liver, blood fat, lowering blood sugar, increase anti-fatigue and anti-fatty liver hematopoiesis such as the In this study, conducted by organic solvent extraction, crude obtained Pigment extraction The results showed that the main alumina crude was purified by chromatography, eluting with petroleum ether and effective fat-soluble impurities in crude,Then petroleum ether - acetone (volume ratio 10:1) as eluent can effectively get rid of carotenoids, using thin layer chromatography (TLC method), high performance liquid chromatography (HPLC method) method, β- carotene, carotenoids of up to 78% purityKey words: Pigment separation and purification of column chromatography