The purpose:According to the physical and chemical properties of shikonin on the total pigment shikonin in Lithospermum to optimize the extraction The method:First of all, with 95%, respectively, ethanol, petroleum ether (60 ℃ -90 ℃), acetone, chloroform and other organic solvents for extraction of total pigment shikonin ability to inspect, the better the selection of solvent extraction, and then choose extraction dynamic orthogonal extraction were optimized in order to Shikonin total extraction rate for the pigment inspection targets, optimization of the extraction of the best Results: the best optimization by orthogonal experiments and extraction of solvent extraction methods, colorimetric assay, statistical analysis to determine the total pigment shikonin the optimum Conclusion: petroleum ether (60 ℃ -90 ℃) as extraction solvent, extraction dynamic, temperature controlled at 50 ℃, plus 10 times the dosage of Health of the solvent, extracting twice an hour extraction, this is Shikonin the best total pigment Key words: Boraginaceae; Shikonin total pigment; extraction process; orthogonal experiment; Colorimetry; dynamic extraction method