从龙胆草中龙胆苦苷的含量看龙胆草药材资源的保护与利用摘 要目的:运用高效毛细管电泳测定不同产地、不同采收期及不同生长方式的龙胆草中龙胆苦苷的含量,为龙胆草的人工栽培提供技术依据。方法:毛细管区带电泳(CZE);运行电解质为含25%甲醇的20mmol·L-1硼砂缓冲液(pH=32);工作电压30KV;柱温25℃;检测波长275nm。结果:测得龙胆苦苷的回归方程为A=0932C-0517,R=99981;平均回收率为64%,RSD=11%。结论:通过对10个不同样品的测定可以看出,不同产地、不同采收期的野生龙胆草中龙胆苦苷的含量有较大差异;栽培品不同生长年限龙胆苦苷的含量与生长年限成正相关,即龙胆苦苷的含量3年生>2年生>1年生。【关键词】:毛细管区带电泳;龙胆草;龙胆苦苷。AbstractGoal: Utilizes the hpce determination different habitat, the different recovery time and in different growth way gentiopicroside’s of Radix Gentianar’s content, provides the technical basis for the Long Dancao manual Method: Free solution capillary zone electrophoresis(CZE)were carried out in a buffer solution of 20 mmol·L -1 borax solution containing 25% methanol(pH =32) ;running voltage was 30 kV;column temperature was 25℃;the detective wavelength was 275 Results:he regression equation of entiopicroside was A=0932-0517(r=99981) ,The average recovery was 6% ;RSD=1%(n=6)Conclusion: Through determinations 10 different samples we could know that the different habitat, in different recovery time wild gentiopicroside’s of Radix Gentianar’s content has a bigger difference; so gentiopicroside’s content 3 years lives> 2 years to live > 1 year to 【Key words】: Free solution capillary zone eiectrophoresis; Radix Gentianar; 目 录摘要……………………………………………………………………1一、 前言……………………………………………………………3二、 实验部分………………………………………………………41、 仪器与试药……………………………………………………42、 方法与结果……………………………………………………43、 讨论……………………………………………………………74、 结论……………………………………………………………8三、 综述部分………………………………………………………101、 来源及植物形态………………………………………………102、 栽培技术………………………………………………………103、 化学成分………………………………………………………124、 药理作用………………………………………………………135、 临床应用………………………………………………………14四、 致谢……………………………………………………………15五、 参考文献………………………………………………………16