The horse streptococci(the Streptococcus equi) belong to the 兰 surname germ for cent of the Cs streptococci, include the horse streptococci MA3 YA3 ZHONG3 (S equi Equi),the horse streptococci monster epidemic disease second kind(S equi Zooepidemicus)with horse streptococci MA3 YA3 ZHONG3 (S equi equisi milis)That germ can pass external injury,surgical operation,or cure the path infect various animals, causing diseases, such as septicemia Sepsis,meningitis Encephalitis and mastitis(Mastitis)Its particular gene expression outcome is pathogenic(Pathogenicity) to germ to have special Study gene, be in order to understand its function and expression and adjust to control a The information of make use of the living creature's learn(Bioinformatics) can adjust to control to carry on the estimate and analysis to the function and expression of the gene, the province goes to a trivial experiment to investigate, providing the instruction direction for the experiment The function and sequence of the gene are closely-related, when the likeness that is a sequence exceeds certain scope, they carry out same function probably, pass will don't know the function sequence with have already known the contrast of the function sequence, if their likeness is higher and then can predict a sequence to correspond The purpose of this topic research is to be learn with the living creature information Be the theories foundation usage tool software with according to WEB on-line service to some a do not know the horse streptococci gene carry on a series of analysis, the result integrate, predicting the function of this gene expression outcome The horse streptococci P50 gene sequence length is a 100 BPs, the whole gene has a to open all of the reading frame(ORF)s to grow a 90 BPs, coding 100 amino acids 残基 The horse streptococci P50 the gene sequence entered the BLAST service net industry that the NCBI provide first, handle stuffs with the form and list of the net industry up, the choice correspond of the parameter hand over to the data server to go forward to act like the source index(the homology Search)Many living creature information centers all have the BLAST server of the specialized Here applied http: v?b last?(Circulate BLASTR0, the United States, the maintenance GenBank)Then the result detection of the basis BLAST index(Search) and the protein of the horse streptococci P50 gene sequence expression together the source is the tallest of 3 kinds of protein one by one in order is 123The secondly applied DNASTAR software translates the horse streptococci P50 gene sequence into the amino acids sequence, the result manifestation P50 gene sequence translates 20 amino acidses Will and the horse streptococci P50 amino acids sequence together source the high sequence inputs to the tool software respectively, carrying on evolving a tree(the Phylogenetic Tree) to Hand over the horse streptococci P50 amino acidses(the Amino Acids) sequence(Sequence) to the professional website in, can immediately predict the second class structure of that amino acids Then make use of a software analysis P50 gene protein physics and chemistry The Darwinian Theory of Evolution(the Evolutionary theory) gave comparison the gene set to learn to provide the theories The relative relation(kinship) is more near, its gene sequence is a source(homology) together more Two sequences together the source is more high, may express the function of the outcome(the Expression Product) very much so more Through the sorting to the Analytical, according to discover the horse streptococci P50 genes express of may be the fat egg white(the Substrate binding Lipoprotein) that a kind of bottom thing combine, participate the metals transport system(the Transport System) to constitute directly, the kind's transport system has in the germ from process acquire nourishment inside the host(HOST) body to close an important This kind of egg white is the fat egg white(the Substrate binding Lipoprotein) that a conveyance,such as the bottom thing of the metals ions, such as iron,manganese and , combine, we can call it as the streptococci metals to combine the fat egg (the Metal binding lipoproteins, MBLS)This kind of egg white is a kind of poisonous dint factor(the virulent factor) of the horse streptococci(the Streptococcus equi) is a germ with the result that the foundation of the disease to the horse streptococci physiology bio-chemical characteristic and pathogenic have very important influence and the